HPLC method for the quantitative determination of sarcophine, a source of cembranoids with cancer chemopreventive activity.

نویسندگان

  • Shymaa El-Shawadfy Bilasy
  • Sherief I Khalifa
  • Samy M Saleh
  • Soad H Abou El-Ela
چکیده

An accurate, reproducible and sensitive method for the quantitative determination of sarcophine in the organic extract of the Red Sea soft coral Sarcophyton species was developed and validated. Sarcophine concentration was determined by RP HPLC using ODS column. The mobile phase was made up of 70% acetonitrile in deionized water and the pH was adjusted to 3.5 with phosphoric acid. The flow rate was 1.5ml/min and the detector was set to 220nm. The HPLC analysis of several Sarcophyton glaucum samples collected from different locations in the Red Sea revealed that Hurghada had the highest sarcophine concentration.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Potent Skin Cancer Chemopreventing Activity of Some Novel Semi-synthetic Cembranoids from Marine Sources

In the course of our continuing research in development and evaluation of novel skin cancer chemopreventive agents from marine sources, five semi-synthetic cembranoids derived from the marine natural product sarcophine, isolated from the soft coral Sarcophyton glaucum, were synthesized and shown to exhibit a remarkable chemopreventive activity in the in-vitro Epstein Barr Virus Early Antigen (E...

متن کامل

Three New Cembranoids from the Taiwanese Soft Coral Sarcophyton ehrenbergi

In order to search for new bioactive substances from marine organisms, we have investigated the acetone extracts of the soft coral Sarcophyton ehrenbergi collected at San-Hsian-Tai, Taitong County, Taiwan. Chromatographic fractionation of the extracts of the octocoral S. ehrenbergi led to the isolation of three new cembranoids, (+)-12-ethoxycarbonyl-11Z-sarcophine (1), ehrenbergol A and B (2 an...

متن کامل

Bioassay Methods useful to select the natural product cancer chemopreventive agents

Prevention of malignant disease such as cancer is obviously much more important than therapy, so primary prevention tactics should be invoked with the greatest possible effort. Because delaying a disease such as cancer is somehow equivalent to cure and it is now generally accepted that cancer can be managed through chemopreventive interventions. Since a considerable number of known cancer chemo...

متن کامل

Bioassay Methods useful to select the natural product cancer chemopreventive agents

Prevention of malignant disease such as cancer is obviously much more important than therapy, so primary prevention tactics should be invoked with the greatest possible effort. Because delaying a disease such as cancer is somehow equivalent to cure and it is now generally accepted that cancer can be managed through chemopreventive interventions. Since a considerable number of known cancer chemo...

متن کامل

Polyoxygenated Cembrane Diterpenoids from the Soft Coral Sarcophyton ehrenbergi

Five new polyoxygenated cembranoids, named (+)-1,15-epoxy-2-methoxy-12-methoxycarbonyl-11E-sarcophytoxide (1), (+)-2-epi-12-methoxycarbonyl-11E-sarcophine (2), 3,4-epoxyehrenberoxide A (3), ehrenbergol D (4) and ehrenbergol E (5), were obtained from the soft coral Sarcophyton ehrenbergi. The structures of 1-5 were established on the basis of comprehensive NMR and HR-ESI-MS analyses and by compa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of pharmaceutical and biomedical analysis

دوره 46 4  شماره 

صفحات  -

تاریخ انتشار 2008